A multiplex-PCR for the differentiation of Mycobacterium bovis and Mycobacterium tuberculosis
نویسندگان
چکیده
منابع مشابه
Multiplex-PCR for differentiation of Mycobacterium bovis from Mycobacterium tuberculosis complex
We evaluated a multiplex-PCR to differentiate Mycobacterium bovis from M. tuberculosis Complex (MTC) by one step amplification based on simultaneous detection of pncA 169 C > G change in M. bovis and the IS6110 present in MTC species. Our findings showed the proposed multiplex-PCR is a very useful tool for complementation in differentiating M. bovis from other cultured MTC species.
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Isolates from suggestive bovine tuberculosis lesions were tested by a multiplex polymerase chain reaction (m-PCR) targeting for RvD1Rv2031c and IS6110 sequences, specific for M. bovis and Mycobacterium tuberculosis complex respectively. The m-PCR successfully identified as M. bovis 88.24% of the isolates.
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26 Tuberculosis (TB) in humans is caused by members of the Mycobacterium 27 tuberculosis complex (MTC). Rapid detection of the MTC is necessary for the timely 28 initiation of antibiotic treatment, while differentiation between members of the 29 complex may be important to guide the appropriate antibiotic treatment and provide 30 epidemiological information. 31 In this study, a multiplex real-t...
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In view of the considerable and continuing public health significance of Mycobacterium bovis infection in humans and animals, WHO convened a meeting on zoonotic tuberculosis in Geneva in November 1993. The participants at the meeting reviewed the human and animal tuberculosis situation worldwide, discussed the zoonotic aspects of M. bovis infection in United Republic of Tanzania and Zambia, exc...
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ژورنال
عنوان ژورنال: FEMS Microbiology Letters
سال: 2002
ISSN: 0378-1097
DOI: 10.1016/s0378-1097(02)00844-3